The Last Guy

You need to crush every single roadblock obstacle on stage B-2. To do that:
1) Run when possible to get further in less time, every second counts. Enemies will flourish in areas if you wait, making it impossible.
2) There is an exact path you must follow, in precisely the right fashion, to get all the obstacles in time.
3) Don't alert any of the walking eyeballs.
4) You will need to rescue practically everyone to be able to crush the obstacles in each section. Also, don't lose anyone in your line.
If you mess up any the above, you aren't going to make it that run.

BattleAxis will show you how to do it in this video: