Black Widow: Recharged

You can get this in Arcade or Challenge Mode. You just need to wait for a Hornet to appear, then wait for it to lay an egg. When it does, you need to push the egg off the outer edge of the web so it falls down into oblivion. It must be the outer edge of the Web, the central hole won't work for this trophy. There's a pretty decent chance you'll get this naturally as some of the Challenges require you to get rid of the eggs before they hatch and unless you have an explosive shot power-up active, pushing is the only way to get rid of them.