Black Widow: Recharged

You'll knock out most of these as you progress through Challenge Mode (if you're struggling through this Mode, there's a possibility you'll get all 2000). If you don't have it by the time you've completed all 30 Challenges, just replay Arcade mode a few times and once you've killed your 2000th overall bug, the trophy will unlock. Do note that the Eggs do not count for this trophy if you destory them before they hatch. The kills are cumulative across solo and coop play.