Botany Manor

Interact with the flush chord above toilet in bathroom accessible after growing Sapphire Gloom, you will have to grow Brook Chalice flower inside bathtub in the same bathroom.

100% complete all achievement guide:

Extended timestamps:
00:00:00 - Chapter 1: A Fresh Start
00:03:25 - "Windmill Wort" Achievement
00:04:30 - "In The Bin" Achievement
00:04:45 - "Green Thumbs" Achievement
00:05:40 - "Take a break" Achievement
00:06:05 - "Take A Nap" Achievement (stay on bench for one minute or more)
00:06:30 - "Let me in!" Achievement

00:07:35 - Chapter 2: Survivors of Adversity
00:08:45 - "Piano Woman" Achievement
00:14:15 - "Boom" Achievement
00:15:15 - "Fulguria" Achievement
00:16:25 - "Crack" Achievement
00:17:00 - "Ash Plume" Achievement
00:17:50 - "Photographer" Achievement

00:19:35 - Chapter 3: Peculiar Petals
00:21:15 - 01/10 Paintings
00:22:05 - "History Sleuth" Achievement
00:23:45 - "Fixer Upper" Achievement
00:28:50 - "Pixie Tears" Achievement
00:30:40 - "Wolfglove" Achievement
00:31:00 - "Mountaineer" Achievement
00:33:55 - "Lockpicker" Achievement

00:34:30 - Chapter 4: Botanical Allies
00:34:40 - "Grandma's Vault" Achievement
00:35:10 - 02-09/10 Paintings
00:37:40 - "Sapphire Gloom" Achievement
00:43:10 - "Clean and Tidy" Achievement
00:43:20 - "Who needs a plumber?" Achievement
00:47:50 - 01/05 Ducks
00:47:55 - "Quack Quack" Achievement
00:49:35 - "Nightfall" Achievement
00:52:20 - "Cradle Fern" Achievement
00:54:25 - "Brook Chalice" Achievement
00:55:50 - 02/05 Ducks
00:56:25 - 03/05 Ducks
00:56:40 - 04/05 Ducks

00:58:35 - Chapter 5: Climbing Up
01:00:25 - 10/10 Paintings
01:00:30 - "Art Lover" Achievement
01:00:40 - "Frogger" Achievement
01:06:35 - 05/05 Ducks
01:06:40 - "Quack Quack Quack Quack!" Achievement
01:10:40 - "Help?" Achievement
01:15:05 - "Botanical Researcher" Achievement
01:18:43 - "Springdance Shrub" Achievement
01:21:30 - "Fool's Emerald" Achievement
01:25:30 - "Oscilette" Achievement
01:25:45 - "Flower Arranging" Achievement
01:27:10 - Ending
01:28:00 - "The End" Achievement
01:28:15 - Subscribe!

Chapter 4

The bathroom is connected to the Master Bedroom on the second floor, accessible after growing the Sapphire Gloom. Simply enter and pull the chain on the toilet to flush it.

Full video walkthrough including all achievements, puzzle solutions, and collectible locations! (Timestamps in YouTube Description)